Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Stat3 Antibody (F-2): sc-8019 to perform Western blotting STAT3

Get tips on using Fenozol to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR

Get tips on using PICK1 siRNA to perform siRNA / miRNA gene silencing Rat - Astrocytes PICK1

Get tips on using Fabp5 siRNA to perform siRNA / miRNA gene silencing Rat - PC12 Fabp5

Get tips on using Vps13a siRNA to perform siRNA / miRNA gene silencing Rat - PC12 chorein

Get tips on using E-cadherin monoclonal antibody (ECCD-2) to perform Western blotting E-cadherin

Get tips on using Mouse Lipocalin-2/NGAL DuoSet ELISA to perform ELISA Mouse - NGAL/LCN2

Get tips on using peSpCas9(1.1)-2×sgRNA (empty, donor) to perform CRISPR Human - Repression SLC7A11