Get tips on using CREB-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC CREB
Get tips on using MEK-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF MEK1
Get tips on using ERK 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF ERK1
Get tips on using ENX-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HaCaT EZH2
Get tips on using Flotillin-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 FLOT1
Get tips on using Dlx-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - A549 DLX2
Get tips on using SnoA/N siRNA (h) to perform siRNA / miRNA gene silencing Human - SW1990 SnoN
Get tips on using Arp2 siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D Arp-2
Get tips on using siRNA TICAM-1 to perform siRNA / miRNA gene silencing Human - HT-29 TICAM-1
I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?
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