Immunohistochemistry Wilms Tumor 1 (WT1) Rabbit

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Get tips on using MUC2 (MRQ-18) Mouse Monoclonal Antibody to perform Immunohistochemistry Human - Muc-2

Products Cell Marque Tissue Diagnostics MUC2 (MRQ-18) Mouse Monoclonal Antibody

Get tips on using Sox-9 Antibody (E-9): sc-166505 to perform Immunohistochemistry Human - SOX9

Products Santa Cruz Biotechnology Sox-9 Antibody (E-9): sc-166505

Get tips on using Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) to perform Immunohistochemistry Human - Dicer1

Products Abcam Anti-Dicer antibody [13D6] - ChIP Grade (ab14601)

Get tips on using Mucin 16 Antibody (C-6): sc-365002 to perform Immunohistochemistry Human - CA125

Products Santa Cruz Biotechnology Mucin 16 Antibody (C-6): sc-365002

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type PANC-1 human pancriatic cancer

Get tips on using Anti-vimentin to perform Immunohistochemistry Vimentin (3B4) - Mouse Human -NA-

Products DAKO Anti-vimentin

Get tips on using Anti-MUC2 antibody (ab76774) to perform Immunohistochemistry Mouse - Muc-2

Products Abcam Anti-MUC2 antibody (ab76774)

Get tips on using Mouse Osteopontin/OPN Antibody to perform Immunohistochemistry Mouse - Spp1/OPN

Products R&D system, Minneapolis, MN, USA Mouse Osteopontin/OPN Antibody

Get tips on using Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Products BioLegend Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody

Get tips on using Anti-p53 Protein, Clone DO7 to perform Immunohistochemistry Human - p53

Products Biogenex Anti-p53 Protein, Clone DO7

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