Get tips on using MUC2 (MRQ-18) Mouse Monoclonal Antibody to perform Immunohistochemistry Human - Muc-2
Get tips on using Sox-9 Antibody (E-9): sc-166505 to perform Immunohistochemistry Human - SOX9
Get tips on using Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) to perform Immunohistochemistry Human - Dicer1
Get tips on using Mucin 16 Antibody (C-6): sc-365002 to perform Immunohistochemistry Human - CA125
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Anti-vimentin to perform Immunohistochemistry Vimentin (3B4) - Mouse Human -NA-
Get tips on using Anti-MUC2 antibody (ab76774) to perform Immunohistochemistry Mouse - Muc-2
Get tips on using Mouse Osteopontin/OPN Antibody to perform Immunohistochemistry Mouse - Spp1/OPN
Get tips on using Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1
Get tips on using Anti-p53 Protein, Clone DO7 to perform Immunohistochemistry Human - p53
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