ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) -NA-

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - MDA-MB-231

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - MDA-MB-231

Get tips on using Rabbit monoclonal [EP1890Y] to ATM (phospho S1981) to perform Immunohistochemistry ATM phospho - Rabbit IgG Human -NA-

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - Rat mesenteric smooth muscle cells

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Tissue - mouse kidney

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Rat vascular smooth muscle cells (vSMCs)

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - rat kidney and pancreas tissue

Get tips on using OxiSelect™ Comet Assay Kit (3-Well Slides) to perform DNA Damage Assay HeLa