Immunohistochemistry ATM phospho

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using pJAP2 to perform Protein Expression Eukaryotic cells - HEK293 AT1R

Get tips on using PL_MmAtf4 to perform Protein Expression Eukaryotic cells - CHO Atf4

Get tips on using hCas9 to perform CRISPR Mouse - Deletion 3T3-L1 ATP7A

Get tips on using lentiCRISPR v2 to perform CRISPR Mouse - Deletion ATDC5 Nfatc1

Get tips on using pX330A-1x3 to perform CRISPR Mouse - Deletion ATDC5 Perk

Get tips on using Mm_Pttg1ip_7 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - AtT20 PTTG1

Get tips on using Mm_Gadd45b_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - AtT20 GADD45β

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Rat - Deletion AR42J Atg12

Get tips on using Stealth siRNA(m)_Atg5 to perform siRNA / miRNA gene silencing Mouse - MLO‐Y4 Atg5