siRNA / miRNA gene silencing Mouse B16 Melanoma cells

Get tips on using Silencer® Select_Alkbh1 siRNA (r) to perform siRNA / miRNA gene silencing Rat - B35 Alkbh1

Get tips on using ON-TARGETplus human ATG16L1 siRNA to perform siRNA / miRNA gene silencing Human - SHSY5Y ATG16L1

Get tips on using SMARTpool: ON-TARGETplus TP63 siRNA to perform siRNA / miRNA gene silencing Human - A253 P36

Get tips on using ON-TARGETplus Human TET1 siRNA to perform siRNA / miRNA gene silencing Human - A172 TET1

Get tips on using B2M siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) B2M

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

Get tips on using Stealth siRNA(r)_Mmp15 to perform siRNA / miRNA gene silencing Rat - C6 (rat glioma) mmp15

Get tips on using Stealth siRNA(r)_Ctnnb1 to perform siRNA / miRNA gene silencing Rat - NPC β-Catenin/Ctnnb1

Get tips on using N-WASP siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D N-WASP

Get tips on using VEGF-D siRNA (h) to perform siRNA / miRNA gene silencing Human - Caki-2 VEGF-D