ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) -NA- Human

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - HT-29

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HT-29

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - MCF-7

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - SH-SY5Y

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - Hep G2

Get tips on using BD Cycletest™ Plus DNA Kit to perform Cell cycle assay human - Hep G2

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - PANC-1

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.