siRNA / miRNA gene silencing Human SUIT-2

Get tips on using NGFR p75 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC NGFR p75

Get tips on using SignalSilence® SAPK/JNK siRNA to perform siRNA / miRNA gene silencing Human - KGN SAPK/JNK

Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include: 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi have been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining efficacy of transduction and shRNA on its target site.

Get tips on using SMARTpool: ON-TARGETplus TP63 siRNA to perform siRNA / miRNA gene silencing Human - A253 P36

Get tips on using AllStars Hs Cell Death siRNA to perform siRNA / miRNA gene silencing Human - U2OS KRAS

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using B2M siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) B2M

Get tips on using N-WASP siRNA (h) to perform siRNA / miRNA gene silencing Human - T47-D N-WASP

Get tips on using EPAS-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HeLa EPAS-1 Lipid

Get tips on using IL-8 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC IL-8 Lipid