Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.
Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized A2780
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized Neuro2a
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized E14Tg2a
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized C2C12
Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized T98G
Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized MDCK
Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized CMT12
Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized Jurkat
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment