Microarray Gene expression arrays Mouse liver tissue

Get tips on using ON-TARGETplus Mouse Mprip (26936) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-SA Mprip

Get tips on using ON-TARGETplus Mouse Casp8 (12370) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-SA Casp8

Get tips on using ON-TARGETplus Mouse Myb (17863) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - NIH-3T3 Myb

Get tips on using ON-TARGETplus Mouse Ddit4 (74747) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - RGC-5 Ddit4

Get tips on using ON-TARGETplus Mouse Eif2ak3 (13666) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - CT26 Perk/Eif2ak3

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How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Get tips on using Tissue or Cell Total Protein Extraction Kit to perform Protein isolation Tissue - Lamb muscle tissue