Protein Expression Eukaryotic cells P. pastoris

Get tips on using Bicinchoninic Acid Kit for Protein Determination to perform Protein quantification Mammalian cells - C2C12

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HeLa

Get tips on using Total Exosome RNA & Protein Isolation Kit to perform Protein isolation Mammalian cells - HeLa

Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform siRNA / miRNA gene silencing Human - U251 cofilin-1 (CFL1)

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - PC-12

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Human podocytes

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Rat microglia