siRNA / miRNA gene silencing Human Jurkat MK2 (MAPK Kinase 2)

Get tips on using IL-8 siRNA (h) to perform siRNA / miRNA gene silencing Human - HUVEC IL-8 Lipid

Get tips on using stealth siRNA GIRK1/KCNJ3 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 GIRK1/KCNJ3

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - HT-1376 GLUT1

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - HT-1376 CD74

Get tips on using siRNA ATX-1 or ENPP2 to perform siRNA / miRNA gene silencing Human - A2780 ATX-1

Get tips on using Pre-designed and validated siRNA against gene IGFBP1 to perform siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody to perform Autophagy assay cell type - HepG2

Get tips on using Anti-PI 3 Kinase p85 alpha antibody [EP380Y] to perform Autophagy assay cell type - HepG2

Get tips on using SIRT1 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) SIRT1