Angiogenesis assay mouse

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Caco-2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Hep G2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Capan-2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay SJSA-1

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HEK 293T

Get tips on using Cytochrome c Releasing Apoptosis Assay Kit to perform Apoptosis assay cell type - SKOV3

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Purified Mouse Anti-SV40 Large T Antigen Clone PAb 101 (RUO) to perform Immunohistochemistry Mouse - SV40

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay MIA PaCa-2

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay MDA-MB-231