dna-quantification-human-pc-3

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Yeast - Saccharomyces boulardii

Get tips on using SMARTpool: siGENOME PAK4 siRNA to perform siRNA / miRNA gene silencing Human - OVCAR-3 PAK4

Get tips on using SignalSilence® FoxO3a siRNA to perform siRNA / miRNA gene silencing Human - Calu-3 FOXO3

Get tips on using SignalSilence® FoxO1 siRNA to perform siRNA / miRNA gene silencing Human - Calu-3 FOXO1

Get tips on using siRNA Junction plakoglobin (SASI_Hs01_00246520) to perform siRNA / miRNA gene silencing Human - BxPC-3 plakoglobin

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - BxPC-3

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using MagAttract HMW DNA Kit (48) to perform DNA isolation / purification Bacteria - Gram negative Klebsiella