Protein Expression Prokaryotic cells M. smegmatis

Get tips on using QIAamp DNA Blood Mini Kit to perform DNA isolation / purification Yeast - Saccharomyces cerevisiae

Get tips on using QIAamp DNA Blood Midi Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Escherichia coli

Get tips on using QIAamp DNA Stool Mini Kit to perform DNA isolation / purification Yeast - Saccharomyces boulardii

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using XcelGen Plant RNA Isolation Mini Kit to perform RNA isolation / purification Plants - Seeds

Get tips on using miRCURY Exosome Serum/Plasma Kit to perform Purification of extracellular vesicles Exosomes - Seminal plasma

Get tips on using RNeasy 96 BioRobot 8000 Kit (12) to perform mRNA / Ribonucleoprotein isolation / purification mRNA

Get tips on using MagAttract HMW DNA Kit (48) to perform DNA isolation / purification Bacteria - Gram negative Klebsiella