Immunofluorscence  53BP1 [H-300] Rabbit

Get tips on using Hoechst 33342 to perform DNA quantification Human - HeLa

Get tips on using HincII NEB#R0103 to perform Restriction Enzymes HincII / HindII

Get tips on using Anti-Hes1 antibody (ab49170) to perform Immunohistochemistry Human - Hes1

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.