siRNA / miRNA gene silencing Human LNCap

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using Ccl2 siRNA to perform siRNA / miRNA gene silencing Rat - Neuronal cells MCP-1

Get tips on using Cxcr4 siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells CXCR4

Get tips on using Jun siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a c-Jun

Get tips on using HuR siRNA to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR Lipid

Get tips on using Stealth siRNA_SPI1 to perform siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1

Get tips on using Rn_Pdcd8_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - H9c2 AIF/Pdcd8

Get tips on using Net siRNA (m) to perform siRNA / miRNA gene silencing Mouse - FL83B Elk3/Net

Get tips on using ATG5 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - NIH-3T3 Atg5

Get tips on using p130 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC Rbl2/p130