Get tips on using Bambi siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 BAMBI
Get tips on using Stk11 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 Stk11
Get tips on using Cxcr4 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 CXCR4
Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - BMDMs Dusp3
Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Dusp3
Get tips on using Fenozol to perform siRNA / miRNA gene silencing Human - HeLa Cdc20
Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - LLC PKN3
Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - HeLa PKN3
Get tips on using NM_001350 to perform siRNA / miRNA gene silencing Human - U2OS DAXX
The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.
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