RNA isolation / purification Cells immortalized

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized LNCaP

Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Cells - immortalized Ku812

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized A2780ADR

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - immortalized CHO

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Rat cell lines

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.