siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven

Get tips on using p130 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC Rbl2/p130

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - MEL-1220

Get tips on using FGD4 siRNAs (FlexiTube) to perform siRNA / miRNA gene silencing Human - LNCap FGD4

Get tips on using Timp2 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 TIMP-2

Get tips on using NFE2L2 siRNA to perform siRNA / miRNA gene silencing Mouse - B16-F10 Nrf2

Get tips on using Kdm2b siRNA to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 Fbxl10

Get tips on using Stk11 siRNA to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 Stk11

Get tips on using MEK-3 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - BMDMs MEK-3

Get tips on using Silencer®_Skiv2l2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - P19 Skiv2l2