rna-isolation-purification-cells-primary-human-carotid-artery-endothelial-cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Dynabeads™ Untouched™ Human T Cells Kit to perform Cell Isolation Human T cells

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized PC-12

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized RIN-5F

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - immortalized KG-1

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Mammalian cell culture media RAOEC

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized Human blood cord

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Human Liver

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human testes