The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Tissue - human brain tissue
Get tips on using AllPrep DNA/RNA Micro Kit to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary mouse cortical neurons
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - adipose
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - eye
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - lung
Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Tissue - brain
Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment