Get tips on using Cxcr4 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 CXCR4
Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - BMDMs Dusp3
Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Dusp3
Get tips on using Gabpb1 siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Nrf2
Get tips on using Ccr2 siRNA to perform siRNA / miRNA gene silencing Rat - Glial cells CCR2
Get tips on using INTERFERin® to perform siRNA / miRNA gene silencing Human - 501 Mel and SK Mel 28 FANCD2 Polymer / Lipid
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
Get tips on using Timp2 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 TIMP-2
Get tips on using NFE2L2 siRNA to perform siRNA / miRNA gene silencing Mouse - B16-F10 Nrf2
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