ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue TGFBI

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - MG-63

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HL-60

Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - HL-60

Get tips on using Q-Plex™ Mouse Cytokine – Inflammation (14-plex) to perform ELISA Mouse - GM-CSF

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using WST-1 Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - MG-63

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Bacillus anthracis

Get tips on using TAGZyme Kit to perform Protein tag His-tag removal