PCR Multiplex PCR

Get tips on using ON-TARGETplus Human PCSK7 (9159) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 IGFBP-7

Get tips on using ON-TARGETplus Human PCSK6 (5046) siRNA - Individual to perform siRNA / miRNA gene silencing Human - Detroit 562 / D562 PACE4

Get tips on using FOXP3 Monoclonal Antibody (PCH101), Alexa Fluor 700, eBioscience™ to perform Flow cytometry Anti-bodies Human - FOXP3

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells HUVEC

Get tips on using RPMI-1640 with Phenol Red produced by FUJIFILM Wako Pure Chemical Corporation to perform Mammalian cell culture media Ku812

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary rat astrocytes

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary human chondrocytes

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using Gentra Puregene Blood Kit to perform DNA isolation / purification Cells - Primary cells CD4+ T cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.