Angiogenesis assay human - HUVEC

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

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2 years ago

2 years ago by Sander J. Mikkelsen Denmark

endothelial cells vs HUVEC cells?

What is the difference between endothelial cells and HUVEC cells for angiogenesis assay?

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Protocol tips
Cells are supplemented with 2% FBS containing SH-SY5Y conditioned media alone or supplemented with 150 ng/ml anti-VEGF or melatonin 1 mM and after incubation for 5 h,tube-like formation was observed by microscopy.
Protocol tips
- 200 µL of thawed ECM gel solution to each well.

- 600 uL of cell suspension was added onto the solidified ECM gel and incubated the assay plate at 37ºC for18 hrs.
Protocol tips
Cells are observed by microscopy after 6h of incubation.
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