Cell cycle assay human - MCF-7

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

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Found 3 matching solutions for this experiment

Muse® Cell Cycle Assay Kit

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
It is important to have a single cell suspension prior to ethanol fixation	Resuspend the cell pellet in 200 μL of Muse™ Cell Cycle Reagent.

Upstream tips
It is important to have a single cell suspension prior to ethanol fixation
Protocol tips
Resuspend the cell pellet in 200 μL of Muse™ Cell Cycle Reagent.

Manufacturer protocol Publication protocol 1 Relevant paper

BD Cycletest™ Plus DNA Kit

BD Biosciences

Upstream tips	Protocol tips	Downstream tips
- After cells are seeded and treated with vernodalin for 24 hours, cells were fixed with 70% ethanol overnight.

Upstream tips
- After cells are seeded and treated with vernodalin for 24 hours, cells were fixed with 70% ethanol overnight.

Manufacturer protocol Publication protocol 1 Relevant paper

Guava Cell Cycle Reagent for Flow Cytometry

Merck Millipore

Upstream tips	Protocol tips	Downstream tips
- Cels were seeded for 24 hours, and incubated in the absence or presence of erucin for an another 24 hours,

Upstream tips
- Cels were seeded for 24 hours, and incubated in the absence or presence of erucin for an another 24 hours,

Manufacturer protocol Publication protocol 1 Relevant paper

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