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1 year ago
1 year ago by Stefan Fuhrmann
Hello everyone. I am in need of some help. After isolating DNA and amplifying it I ran it through an agarose gel to check it. When I tried to extract it from the gel the amount of DNA I got was too low to use. For instance, I started with around 500 ng/μl but managed to extract only concentrations as low as 10 ng/μl. What might be causing this significant loss? Also, I am wondering if purifying the pcr product with PEG mix is sufficient.
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Thermo Fisher Scientific