DNA isolation / purification Yeast - Saccharomyces cerevisiae

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Start discussion

Found 1 discussion for this experiment

Discussion

5 years ago

5 years ago by Jean Rossignol France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

Share your thoughts or question with experts in your field by adding a discussion!

Found 4 matching solutions for this experiment

Downstream tips
- Include RNAse treatment for 15-20 min.
- Ensure EtOH is completely evaporated off of the column prior to elution. Adjust time from 1min to 5 min at 60`C
- Use prewarmed TE buffer to elute the DNA
Protocol tips
Susceptibility to yeast lytic enzymes varies for different yeast
species.

If you see incomplete lysis, extend the first incubation time up to 2 hours or over 16 hours.
Upstream tips
DO NOT add bleach or acidic solutions directly to the sample-preparation
waste.
Downstream tips
- Include RNAse treatment for 15-20 min.
- Use prewarmed TE buffer to elute the DNA
Downstream tips
- Include RNAse treatment for 15-20 min.
- Use prewarmed TE buffer to elute the DNA
Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms