ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

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Found 2 matching solutions for this experiment

Mouse ANGPTL3 ELISA

Raybiotech

Upstream tips	Protocol tips	Downstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.	-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.	-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Upstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.
Protocol tips
-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.
Downstream tips
-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Manufacturer protocol Publication protocol 1 Relevant paper

ANGPTL3 (mouse/rat) Dual ELISA Kit

AdipoGen Lifesciences

Upstream tips	Protocol tips	Downstream tips
-Plate and reagents should be at room temperature before use. -Do not expose reagents to temperatures greater than 25°C.	- Prepare just the appropriate amount of the buffers necessary for the assay. -Avoid freeze/thaw cycles. -Keep TMB Substrate Solution (TMB) protected from light.	-The diluted STREP-HRP is not stable and cannot be stored. -The reconstituted standard is aliquoted and stored at -20°C.

Upstream tips
-Plate and reagents should be at room temperature before use. -Do not expose reagents to temperatures greater than 25°C.
Protocol tips
- Prepare just the appropriate amount of the buffers necessary for the assay. -Avoid freeze/thaw cycles. -Keep TMB Substrate Solution (TMB) protected from light.
Downstream tips
-The diluted STREP-HRP is not stable and cannot be stored. -The reconstituted standard is aliquoted and stored at -20°C.

Manufacturer protocol Publication protocol 1 Relevant paper

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