Live / Dead assay mammalian cells - GH3

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 3 matching solutions for this experiment

Cell Counting Kit-8

Dojindo

Upstream tips	Protocol tips	Downstream tips
Pre-incubate the plate in a humidified incubator (e.g., at 37°C, 5% CO2)	Add CCK-8 solution and incubate the plate for 4 hours in the incubator	. Measure and subtract the O.D. at 600 nm or higher from that of sample if there is a high turbidity in the cell suspension

Upstream tips
Pre-incubate the plate in a humidified incubator (e.g., at 37°C, 5% CO2)
Protocol tips
Add CCK-8 solution and incubate the plate for 4 hours in the incubator
Downstream tips
. Measure and subtract the O.D. at 600 nm or higher from that of sample if there is a high turbidity in the cell suspension

Manufacturer protocol Publication protocol 1 Relevant paper

CellTiter-Glo® Luminescent Cell Viability Assay

Promega

Upstream tips	Protocol tips	Downstream tips
	Add reagent and mix contents for 2 minutes to induce cell lysis. Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal.

Protocol tips
Add reagent and mix contents for 2 minutes to induce cell lysis. Allow the plate to incubate at room temperature for 10 minutes to stabilize luminescent signal.

Manufacturer protocol Publication protocol 1 Relevant paper

EZ4U - Cell Proliferation Assay

Biomedica Immunoassays

Upstream tips	Protocol tips	Downstream tips
	Add 200 µl cell culture into respective wells. Add 20 µl SUB (substrate) into each well, swirl gently. Incubate at 37°C for 2-5 hours,

Protocol tips
Add 200 µl cell culture into respective wells. Add 20 µl SUB (substrate) into each well, swirl gently. Incubate at 37°C for 2-5 hours,

Manufacturer protocol Publication protocol 1 Relevant paper

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