Mammalian cell culture media DU145

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

Start discussion

No discussions found

Share your thoughts or question with experts in your field

Start a discussion

Found 3 matching solutions for this experiment

RPMI 1640, with L-Glutamine and 25mM HEPES

Lonza

Upstream tips	Protocol tips	Downstream tips
	RPMI-1640 (BE12-167F; Lonza) was supplemented with fetal bovine serum (10270106; Thermo Fisher Scientific), AB-serum (H4522; Sigma-Aldrich) where indicated, 100 U/ml penicillin, 100 mg/ml streptomycin (DE17-603E; Lonza), 2 mmol/l l-glutamine (BE17-605E; Lonza), 25 mmol/l HEPES and 1 mmol/l sodium pyruvate

Protocol tips
RPMI-1640 (BE12-167F; Lonza) was supplemented with fetal bovine serum (10270106; Thermo Fisher Scientific), AB-serum (H4522; Sigma-Aldrich) where indicated, 100 U/ml penicillin, 100 mg/ml streptomycin (DE17-603E; Lonza), 2 mmol/l l-glutamine (BE17-605E; Lonza), 25 mmol/l HEPES and 1 mmol/l sodium pyruvate

Manufacturer protocol Publication protocol 1 Relevant paper

Gibco™ RPMI 1640 Medium

Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA)

Protocol tips
supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA)

Manufacturer protocol Publication protocol 1 Relevant paper

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

Sigma-Aldrich

Upstream tips	Protocol tips	Downstream tips
	supplemented with 10% FBS and antibiotics (all from Sigma, St. Louis, MO)

Protocol tips
supplemented with 10% FBS and antibiotics (all from Sigma, St. Louis, MO)

Manufacturer protocol Publication protocol 1 Relevant paper

Can't find the product you've used to perform this experiment? It would be great if you can help us by Adding a product!

Fill out your contact details and receive price quotes in your Inbox