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1 year ago
1 year ago by Jesualdo Hernández
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
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|Cells were grown in 6-well plates until subconfluence and then exposed to NTHi lysates for the times indicated. Cells were washed with phosphate-buffered saline and then fractionated using nuclear and cytoplasmic extraction reagents (NE-PER; Pierce Biotechnology) according to the manufacturer’s recommendations.|