Found 2 discussions for this experiment
2 years ago
2 years ago by Paul G. Macon
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
3 years ago
3 years ago by Aaron Stege
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Found 3 matching solutions for this experiment
|- To allow complete penetration by formalin, use tissue samples less than 5 mm thick. If buffers are precipitated dissolve them by heating to 70°C with gentle agitation
|- Perform all centrifugation steps using a microcentrifuge placed at 15–25°C
|- Do not exceed fixation time of 24 hours as it results in poor performance in downstream assay
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