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Found 3 matching solutions for this experiment
| Upstream tips |
Protocol tips |
Downstream tips |
- 5×104 cells/well were seeded in 6 well plate.
- Cells were pretreated with DPT at different time intervals. |
- Cells were incubated with DCFH-DA or MitoSOX at a final concentration of 10 μM or 5 μM for 15 min at 37°C |
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| Upstream tips |
- 5×104 cells/well were seeded in 6 well plate.
- Cells were pretreated with DPT at different time intervals. |
| Protocol tips |
| - Cells were incubated with DCFH-DA or MitoSOX at a final concentration of 10 μM or 5 μM for 15 min at 37°C |
| Upstream tips |
Protocol tips |
Downstream tips |
| - 2.5 × 105 were seeded. |
- Treated with DCFH-DA for 1 h at 37°C.
- After DCFH-DA treatement, cells were treated with Vitisin A (4 µM) and/or TRAIL (20 ng/ml) for 1 h at 37°C |
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| Upstream tips |
| - 2.5 × 105 were seeded. |
| Protocol tips |
- Treated with DCFH-DA for 1 h at 37°C.
- After DCFH-DA treatement, cells were treated with Vitisin A (4 µM) and/or TRAIL (20 ng/ml) for 1 h at 37°C |
| Upstream tips |
Protocol tips |
Downstream tips |
|
- Cells were stained and incubated for 30 min. at 37°C in the dark. |
|
| Protocol tips |
| - Cells were stained and incubated for 30 min. at 37°C in the dark. |
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