ROS assay cell type - PC-3 human prostate adenocarcinoma

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Upstream tips
- 5×104 cells/well were seeded in 6 well plate.

- Cells were pretreated with DPT at different time intervals.
Protocol tips
- Cells were incubated with DCFH-DA or MitoSOX at a final concentration of 10 μM or 5 μM for 15 min at 37°C
Upstream tips
- 2.5 × 105 were seeded.
Protocol tips
- Treated with DCFH-DA for 1 h at 37°C.

- After DCFH-DA treatement, cells were treated with Vitisin A (4 µM) and/or TRAIL (20 ng/ml) for 1 h at 37°C
Protocol tips
- Cells were stained and incubated for 30 min. at 37°C in the dark.
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