ROS assay cell type - T47D

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

MarkerGene™ Live Cell Fluorescent Reactive Oxygen Species Detection Kit

Marker Gene Technologies

Upstream tips	Protocol tips	Downstream tips
	- 20µM DCFH-DA was treated on cells and incubated at 37°C for 45 min. - Shikonin added to wells at concentration 5µM to generate ROS for 4 Hours.

Protocol tips
- 20µM DCFH-DA was treated on cells and incubated at 37°C for 45 min. - Shikonin added to wells at concentration 5µM to generate ROS for 4 Hours.

Manufacturer protocol Publication protocol 1 Relevant paper

OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

Cell Biolabs

Upstream tips	Protocol tips	Downstream tips
- 10,000 cells were seeded in a 96 well plate. - Cells were starved with serum free medium for 24h before the assay

Upstream tips
- 10,000 cells were seeded in a 96 well plate. - Cells were starved with serum free medium for 24h before the assay

Manufacturer protocol Publication protocol 1 Relevant paper

CM-H2DCFDA (General Oxidative Stress Indicator)

Thermo Fisher Scientific

Upstream tips	Protocol tips	Downstream tips
- Do not dissolve products until immediately prior to use	- Cells were measure for ROS levels after 24h of platting.

Upstream tips
- Do not dissolve products until immediately prior to use
Protocol tips
- Cells were measure for ROS levels after 24h of platting.

Manufacturer protocol Publication protocol 1 Relevant paper

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