ROS assay cell type - T47D

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

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Found 3 matching solutions for this experiment

Protocol tips
- 20µM DCFH-DA was treated on cells and incubated at 37°C for 45 min.

- Shikonin added to wells
at concentration 5µM to generate ROS for 4 Hours.
Upstream tips
- 10,000 cells were seeded in a 96 well plate.

- Cells were starved with serum free medium for 24h before the assay
Upstream tips
- Do not dissolve products until immediately prior to use
Protocol tips
- Cells were measure for ROS levels after 24h of platting.
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