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Found 3 matching solutions for this experiment
|- 20µM DCFH-DA was treated on cells and incubated at 37°C for 45 min.
- Shikonin added to wells
at concentration 5µM to generate ROS for 4 Hours.
|- 10,000 cells were seeded in a 96 well plate.
- Cells were starved with serum free medium for 24h before the assay
|- Do not dissolve products until immediately prior to use
|- Cells were measure for ROS levels after 24h of platting.
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