When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary porcine coronary artery smooth muscle cells
Get tips on using PrimaPure Porcine Endothelial Cells Growth Medium to perform Mammalian cell culture media PAOEC
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery endothelial cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine coronary artery endothelial cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary bovine coronary artery endothelial cells
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human coronary artery endothelial cells
Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary canine coronary artery smooth muscle cells
Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells
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