wound-healing-assay-cell-type-human-a375

Get tips on using Oris™ Pro Cell Migration Assay to perform Wound healing assay cell type - human MDA-MB-231

Get tips on using Oris™ Cell Migration Assay - Fibronectin Coated to perform Wound healing assay cell type - human Caco-2

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human MEWO

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human HUVEC

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - mouse MS1

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - mouse 3T3-L1

Get tips on using IncuCyte® Cell Migration Kit to perform Wound healing assay cell type - human MCF-10A

Get tips on using Oris™ Universal Cell Migration Assembly Kit, 96 wells to perform Wound healing assay cell type - human MCF-7

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.