Analysis of DNA fragments

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - U87

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - FaDu

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HaCaT

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - SKOV3

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HT-29

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - BxPC-3

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - Hep G2

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - PANC-1

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.