ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
Get tips on using Mouse TGF beta 1 ELISA Kit (ab119557) to perform ELISA Mouse - TGF-beta 1
Get tips on using Mouse IL-1 beta ELISA Kit (ab100704) to perform ELISA Mouse - IL-1 beta
Get tips on using Mouse ICAM-1/CD54 Quantikine ELISA Kit to perform ELISA Mouse - ICAM-1/CD54
Get tips on using Mouse Serpin E1/PAI-1 DuoSet ELISA to perform ELISA Mouse - Serpin E1/PAI-1
Get tips on using Mouse C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Mouse - C-Reactive Protein/CRP
Get tips on using Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Rat - OPN
Get tips on using Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit to perform ELISA Rat - ANGPT2
Get tips on using Mouse Retinol Binding Protein 4 ELISA Kit (ab202404) to perform ELISA Mouse - RBP4
Get tips on using Mouse MCP-1 PicoKine™ Fast ELISA Kit to perform ELISA Mouse - MCP1
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment