siRNA / RNAi /miRNA transfection Human Lung Adenocarcenoma (A549/LTEP-a-2)

Get tips on using BrdU Cell Proliferation Assay to perform Cell cytotoxicity / Proliferation assay cell type - MDA-MB-231 breast adenocarcenoma

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - MDA-MB-231 breast adenocarcenoma

Get tips on using Cell Proliferation ELISA, BrdU to perform Cell cytotoxicity / Proliferation assay cell type - MDA-MB-231 breast adenocarcenoma

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Get tips on using MISSION® esiRNA_ human CCL2 to perform siRNA / miRNA gene silencing Human - U251 CCL2

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Human astrocytes

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Human - Precision cut lung slices Expression array

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.