shRNA gene silencing Rat WKY

Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - RAW264.7 CD98

Get tips on using Mm_Hdac5_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 HDAC5

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32

Get tips on using Fenozol to perform siRNA / miRNA gene silencing Human - HeLa Cdc20

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - LLC PKN3

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - HeLa PKN3

Get tips on using NM_001350 to perform siRNA / miRNA gene silencing Human - U2OS DAXX

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.