Get tips on using Tlr4 Mouse siRNA Oligo Duplex to perform siRNA / miRNA gene silencing Mouse - BV2 TLR4
Get tips on using ON-TARGETplus Mouse Lgals3 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 LGAL3S3
Get tips on using ON-TARGETplus Mouse Samhd1 siRNA to perform siRNA / miRNA gene silencing Mouse - BMDMs SAMHD1
Get tips on using ON-TARGETplus Mouse Stat3 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 STAT3
Get tips on using ON-TARGETplus Mouse Prkaa1 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Prkaa1
Get tips on using ON-TARGETplus Rat Fyn siRNA to perform siRNA / miRNA gene silencing Rat - Schwann cells Fyn
Get tips on using Silencer® Select_ MYD88 to perform siRNA / miRNA gene silencing Human - A375 MYD88
Get tips on using Silencer® Select_ TRIF to perform siRNA / miRNA gene silencing Human - A375 TRIF
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
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