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siRNA / miRNA gene silencing

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The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

RNA siRNA / RNAi /miRNA transfection Rat A-10 Cationic lipid based
Fenozol Product

Get tips on using Fenozol to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR

Products A&A Biotechnology Fenozol
SIHK1738 Product

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - Colon-26 PKN3

Products Sigma-Aldrich SIHK1738
s15827 Product

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - IMR-90 CDC7

Products Thermo Fisher Scientific s15827
SIHK1738 Product

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - PANC-1 PKN3

Products Sigma-Aldrich SIHK1738
s15827 Product

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - PANC-1 CDC7

Products Thermo Fisher Scientific s15827
SLC29A4 Product

Get tips on using SLC29A4 to perform siRNA / miRNA gene silencing Human - PANC-1 hENT1

Products Dharmacon SLC29A4
s15827 Product

Get tips on using s15827 to perform siRNA / miRNA gene silencing Human - Capan-1 CDC7

Products Thermo Fisher Scientific s15827
SLC29A4 Product

Get tips on using SLC29A4 to perform siRNA / miRNA gene silencing Human - Capan-1 hENT1

Products Dharmacon SLC29A4
s4215 Product

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - SW1990 DNMT1/3b

Products Thermo Fisher Scientific s4215

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