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Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - Mouse Lung

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - Human Adrenal glands

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - mouse lung tissue

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - mouse brain tissue

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Tissue - mouse liver tissue

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Bacteria - Gram negative Haemophilus influenzae

Get tips on using Total RNA Purification Kit to perform RNA isolation / purification Bacteria - Gram negative Hemophilus influenzae

Get tips on using EZ-RNA Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Brain

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells