ELISA (kit) Human Serum Cytokine measurements (Multiplex assay)

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Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Products Sigma-Aldrich In Situ Cell Death Detection Kit, TMR red

Get tips on using Oris™ Cell Migration Assay to perform Wound healing assay cell type - human MCF-10A

Products Platypus Technologies Oris™ Cell Migration Assay

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - SH-SY5Y human neuroblastoma

Products Promega ROS-Glo™ H2O2 Assay

A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.

Cellular assays Angiogenesis assay mouse MS1

Get tips on using Oris™ Pro Cell Migration Assay to perform Wound healing assay cell type - human HUVEC

Products Platypus Technologies Oris™ Pro Cell Migration Assay

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Products Sigma-Aldrich In Situ Cell Death Detection Kit, Fluorescein

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - A549, NCI-H460, H1299 human lung cancer cells

Products Sigma-Aldrich In Situ Cell Death Detection Kit, TMR red

Get tips on using ApopTag® Fluorescein In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - A549, NCI-H460, H1299 human lung cancer cells

Products Millipore ApopTag® Fluorescein In Situ Apoptosis Detection Kit

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - PANC-, BxPC-3 human pancreas

Products Promega ROS-Glo™ H2O2 Assay

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase BHK-21 baby hamster kidney cells

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