Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human RT-7
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human BxPC-3
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human epidermal keratinocytes
Get tips on using Oris™ Cell Migration Assay - Fibronectin Coated to perform Wound healing assay cell type - human Caco-2
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human Caco-2
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human MCF-7
Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line
Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma
A standard angiogenic assay involves the autonomous endothelial cell response of self-organization into microvessels, also known as tubes when seeded on a basement membrane matrix in the presence of the appropriate growth factors. However, the component of basement membrane matrix may also affect the tube formation by endothelial cells. Hence it is important to use a standard angiogenesis assay kit or use the same membrane matrix with known composition to standardize the assay conditions.
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