Protein expression and purification

Get tips on using BLOCK-iT™ Adenoviral RNAi Expression System, pAd/BLOCK-iT™-DEST RNAi Gateway Vector to perform shRNA gene silencing Mouse - P19 Foxm1

Get tips on using LowCell# ChIP kit protein A x16 to perform ChIP Human - HepG2

Get tips on using Mouse C Reactive Protein ELISA Kit (PTX1) (ab157712) to perform ELISA Mouse - C-Reactive Protein/CRP

Get tips on using Mem-PER™ Plus Membrane Protein Extraction Kit to perform Protein isolation Mammalian cells - Mouse_Brown fat

Get tips on using PRO-PREP™ Protein Extraction Solution (C/T) to perform Protein isolation Mammalian cells - 3T3-L1

Get tips on using Quick Start™ Bradford Protein Assay Kit 1 to perform Protein quantification Mammalian cells - BV-2

Get tips on using illustra tissue and cells genomicPrep Mini Spin Kit to perform DNA isolation / purification Tissue - kidney

Get tips on using Rat Bone Morphogenetic Protein 2 ELISA to perform ELISA Rat - BMP-2

Get tips on using PRO-PREP™ Protein Extraction Solution (C/T) to perform Protein isolation Mammalian cells - Mouse Epididymal fat

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase